Masamba a PCR omwe amapangidwa ndi yongyue Medical nthawi zambiri amagawidwa mbale za pcr, semi skided pcr mbale ndi mbale zokhala ndi siketi yonse. Pulogalamu yopanda siketi: yogwirizana ndi matekitala ambiri owombera kapena makina a QPCR, koma osakhala oyenera kugwiritsa ntchito makina okha. Kukhazikika sikuli kukwera nthawi pakupukutira, ndipo ayenera kugwiritsidwa ntchito ndi wogwira mbale. Semu sturded PCR Pulogalamu ya PCR: Itha kusinthidwa chifukwa cha kulembapo kapena kugwiritsa ntchito varcode ntchito, ndi yoyenera kugwiritsa ntchito kokha ndipo imakhala ndi chipika chabwino. Makina okwanira PCr PCR: bwino kwambiri pamapulogalamu a labotale okha, komanso oyenerera malembedwe ndi ntchito zogwirira ntchito. Mphamvu zabwino zamakina, zoyenera zida za PCR yokhala ndi ma module otuluka, komanso kukhazikika kwambiri pakamaso. Kwa 96 pcr mbale kapena 384 mbale ya PCR, ngodya zodulidwa ndi zolembera zimakhazikitsidwa chifukwa cha ngodya za odulidwa ndi zilembo. Kudula ngodya: Kusankha kwa makona odulidwa a PCr Plate kumadalira zofunikira za chida chofananacho, chomwe chitha kukhala bwino.

Kuzindikira: Kulemba kwa alphanumeric kwa pcr kumathandizira kuzindikira zitsime ndi malo osungirako. Izi nthawi zambiri zimakhala choloweza chinsinsi cha chinsinsi kapena logo lolemba. Poyesera zina zofunsira agwiritsi ntchito, mtengo wa mumbale umasindikizidwa bwino ndi zolembedwa zolembedwa.

①why ndi ma pcr opanga omwe amapangidwa kuchokera ku PP?

Chifukwa ma pcr / qpcr zosemphana nthawi zambiri amalumikizana mwachindunji ndi ma reagents kapena a polyplene (ma pp) ndi zinthu zabwino zomwe zimasungidwa mosavuta ndikusinthasintha kwa mankhwala a 121 ° C komanso timalimbananso ndi kutentha pafupipafupi pakadutsa njinga ya kutentha).

Ngati voliyumu yachitsanzo ndi yaying'ono, chubu chimodzi kapena chubu chimakondedwa. Komabe, kuchuluka kwa kuchuluka kwakukulu kwamphamvu, chubu chimodzi ndi chachikulu, ndipo voliyumu imatha kukhala 0,5 ml. Chivindikiro chathyathyathya ndi cowvex chivid chili ndi zabwino zawo. Chonde onani chidziwitso chotsatirachi.

Chubu chimodzi: 0.2ml kapena 0,5ml, kusankha kosasinthika kwa bisipre.0 ml pcr matisi.0.5 ml pcr machubu.

PCR Tuzani machubu: 0.2ml kapena 0.15ml, 8 strip pcr mapira kapena machubu 12

Chipilala chathyathyathya: chimaperekanso kufalitsa kwaulere kwa QPCR ndikuthandizira kulemba ndi kulemba

Chophimba cha Convex: Chimalumikizana ndi chivundikiro cha chophimba cha PCR kuti muchepetse kuwonongeka kwa acti. Koma zimakhudza kufalikira kwa zizindikiro za fluorescent ndipo sizingagwiritsidwe ntchito poyesa QPCR.

② Kodi chifukwa chiyani pcr pctel villas ikutuluka ndi ena? Ndi uti wabwino?

Amene amakwanira ndiye wabwino kwambiri. Pali mitundu iwiri ya pcr wamba PCR: Flat Ellges ndikukweza m'mphepete. Mbale yokhala ndi zitsime zathyathyathya bwino kwambiri. Mphepete imachitika zisindikizo pachitsime chosindikizira mosavuta, kuchepetsa chiopsezo chowodzera pakati pa zitsanzo.

Kodi mungasankhe maketi a pcr ndi mabatani osiyanasiyana?

Cholinga chosankhidwa: Kusankha chinthu choyenera malinga ndi zofunikira zoyeserera. Kuchuluka kwa machubu ambiri a PCR kungakwaniritse zosowa za PCR. Kutengera izi, tikulimbikitsidwa kugwiritsa ntchito machubu ang'onoang'ono ochepera. Kusintha kwamphamvu ndikuchepetsa kusintha kwa phokoso chifukwa cha nsonga zazing'ono za mtengo wotsika machubu / mbale. Nthawi yomweyo, chisamaliro chiyenera kumwedwa kuti musawonjezere zochuluka kapena zochepa powonjezera zitsanzo. Zochuluka kwambiri zimatha kuchititsa kuti mawonekedwe a matenthedwe, oyipitsidwa ndi kuipitsidwa, pomwe pang'ono zimatha kuyambitsa kutaya kwake.

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