utoto wa utoto wamagazi

1. Zofala za Serum flum red
Kutolera magazi kudzakhala ndi zowonjezera, palibe anticoagulant kapena procoagant zosakaniza, zongobisa. Amagwiritsidwa ntchito ngati ma biecochemin a routine seramu ndi mayeso okhudzana ndi serology, zoyesedwa zosiyanasiyana zazomwe zimayesedwa komanso ziyeso za syphilis, ziwerengero za hepatitis B zikuluzikulu. Mtundu wa kukonzekera kwa fanizo ndi seramu. Mwazi utakokedwa, umayikidwa mu 37 ° C Kusamba kwa madzi oposa mphindi 30, centrifted, ndipo seramu yapamwamba imagwiritsidwa ntchito pogwiritsa ntchito pambuyo pake.
2. Machubu operekera machubu a seramu mwachangu
Pali coagulant m'magazi osonkhanitsa magazi kuti ifulumize njira yolumikizira. The seru yofulumira ya seru yamkati imatha kuphatikiza magazi omwe adasungidwa mkati mwa mphindi 5. Ndioyenera pakuyesa kwa zochitika mwadzidzidzi. Ndiwo ma coucula ophatikizira kwambiri a biochemin tsiku lililonse, chitetezo, seramu, mahomoni pambuyo pa magazi atakokedwa, kuteteza komanso kusakaniza nthawi 5-8. Matenthedwe akakhala otsika, amatha kuyikidwa mu madzi a 37 c Sambala kwa 10-20min, ndipo seramu kumtunda kumatha kukhala centriffaget pogwiritsa ntchito pambuyo pake.
3. The Golden Cap of the baller Crudetion Gel Custorator Mour Cuber wopanga ku China
Kulekanitsa gel gel ndi mphaka zowonjezeredwa ku chubu chosungira magazi. Zolinga zake zimakhala zokhazikika kwa maola 48 pambuyo pa centrifugation. Procoagulants imatha kuyambitsa makina ophatikizira ndikuthandizira njira yolumikizira. Mtundu wa mtundu wokonzekera ndi seramu, womwe ndi woyenera pakuyesa kwa mafayilo am'madzi am'madzi ndi pharmacokinetical. Pambuyo posonkhanitsa, kuteteza komanso kusakaniza nthawi 5-8, kuyimirira kwa 20-30 min, ndi centrifuge Humnanatance kuti mugwiritse ntchito pambuyo pake.
4. sodium citrate esr kuyesa chubu chakuda
Soluum citrate yolingana ndi mayeso a ESR ndi 3.2% (yofanana ndi 0.109 mol / l), ndi kuchuluka kwa anticoagulant kwa magazi ndi 1: 4. Muli ndi 0.4ml ya 3.8% sodium citrate, ndikujambula magazi ku 2.0ml. Ichi ndi chubu choyesera chapadera cha erryyrocyte shetmentation. Mtundu wachitsanzo ndi plasma, womwe ndi woyenera ESR. Kujambula kwa magazi pambuyo pa zojambula zamagazi, kutchera ndikusakaniza 5-8 kawiri. Gwedezani bwino musanagwiritse ntchito. Kusiyana pakati pake ndi chubu choyesera kuti chiyesedwe chazophatikizira ndikuti ndende ya anticoagant ndiyosiyana ndi kuchuluka kwa magazi, komwe sikuyenera kusokonezedwa.
5. sodium citrate yoyesera chubu yopepuka ya buluu
Sodium citrate makamaka imagwira ntchito ngati anticoagulant mwa kubera calcium ma aons zitsanzo za magazi. Kuzunzidwa kwamphamvu yolimbikitsidwa ndi Komiti Yadzikoli kwa Committeirization ya zamankhwala ndi 3.2% kapena 3.109Mol / l kapena l), ndi kuchuluka kwa anticoagulant kwa magazi ndi 1.1. Thuti yosungirako magazi a vacuum ili ndi 0,2 ml ya 3.2% sodium citrauum anticoagulant, ndipo magazi amasonkhanitsidwa mpaka 2.0 ml. Mtundu wa kukonzekera kwa fanizo ndi magazi athunthu kapena magunkhune. Mukangosonkhanitsa, kudzera munthawi ya 5-8. Pambuyo pa centrifugation, tengani ma gys am'mwamba kuti agwiritse ntchito. Oyenera kuyesa kuyesa, pt, aptt, kuphunzitsa kogwirizana.
6. heparin anticoagi olde ndi chipewa chobiriwira
Heparin adawonjezeredwa ku chubu chosungira magazi. Heparin mwachindunji ali ndi mphamvu ya antithrombin, yomwe imatha kupitirira nthawi yovuta ya fanizoli. Pazoyesera zadzidzidzi komanso zoyeserera zambiri, monga chiwindi ntchito, impso, shuga wamagazi, etc. yoyenera kuyesa kwamagazi. Heparin yochulukirapo imatha kuyambitsa kuphatikizika kwa maselo oyera ndipo sangagwiritsidwe ntchito kwa maselo oyera. Komanso sioyenera kuyigawidwa kwa leukocyte chifukwa imatha kupangitsa kuti mafayilo owoneka bwino ndi buluu wopepuka. Itha kugwiritsidwa ntchito chifukwa cha chiwerewere magazi. Mtundu wachitsanzo ndi plasma. Pambuyo pa zosonkhanitsa magazi, kuteteza ndi kusakaniza kwa nthawi 5-8, ndipo tengani gysma wa kumtunda.
7. Kuwala kobiriwira kobiriwira kwa plasma kupatukana
Kuphatikiza heparin lifiaagulant kwa chikho cha mphira kumatha kukwaniritsa cholinga cholekanitsidwa mwamphamvu ma proma, chomwe ndi chisankho chabwino kwambiri pakuyesa kwa Electrolyte. Pazoyesera zadzidzidzi komanso zoyeserera zambiri, monga chiwindi ntchito, ntchito impso, shuga wamagazi, etc. zitsanzo za ma plsma zimatha kukhala zokhazikika paphirikali 48 mu firiji. Itha kugwiritsidwa ntchito chifukwa cha chiwerewere magazi. Mtundu wachitsanzo ndi plasma. Pambuyo pa zosonkhanitsa magazi, kuteteza ndi kusakaniza kwa nthawi 5-8, ndipo tengani gysma wa kumtunda.

8. Potaziyamu oxalate / sodium fluoride imvi
Sodium fluoride ndi anticoagulant, yomwe nthawi zambiri imagwiritsidwa ntchito kuphatikiza ndi potaziyamu oxalate kapena sodio ndi gawo limodzi la sodium ndi magawo atatu a potaziyamu oxalate. 4Mg mwa osakaniza awa amatha kupanga 1Ml ya magazi osagwirizana ndi masiku 23 ndikuletsa kuwonongeka kwa shuga. Sizingagwiritsidwe ntchito kutsimikiza kwa urea ndi njira ya urease, kapena kuti musathe kupenya ma phosphata ndi amylase. Ndikulimbikitsidwa chifukwa choyesera magazi. Ili ndi sodium fluoride kapena potaziyamu oxalate kapena dinani la dinani la dinani Pambuyo pokoka magazi, kutchera ndikusakaniza 5-8 kasanu. Plasma yamadzimadzi imasungidwa kuti igwiritsidwe ntchito, ndipo ndi chubu chapadera cha kuchuluka kwa shuga wamagazi.
9. Edta anticoagulation chubu chofiirira chofiirira
Ethylediaminetraacetraocettic acid (edta, molecular kulemera 292) Ndipo mchere wake ndi makonda a heatboxyy, ndipo mayeso a hemoglobin, ndi mayesero a gulu. Osayenera kuyesedwa kwa mayeso ophatikizika ndi mayeso a ntchito, kapena kuti musayenetse calcium ion, potaziyamu ion, ma rosuline phosphatida, alkinine kinasete ndi leucine aminoptidase, oyenera mayeso a PCR. Spray 100ml wa 2.7% Edta-K2 yankho la khoma lamkati la vacuum, lizani magazi a 45 ° C, sonkhanitsani magazi mpaka 2-8 nthawi yomweyo ngati magazi atuluke. Mtundu wachitsanzo ndi magazi athunthu, omwe akuyenera kusakanikirana kwambiri musanagwiritse ntchito.