Kodi tiyenera kusamalira chiyani mukamagwiritsa ntchito mbale za Petri?

Chakudya cha petri ndi chotengera chaboratory chimagwiritsidwa ntchito kuti chikule tizilombo kapena zikhalidwe zam'manja. Imakhala ndi maziko owoneka bwino owoneka bwino komanso chivindikiro. Zinthu za mbale ya petri imagawika m'magulu awiri, makamaka pulasitiki ndi galasi. Zikhalidwe zobzala, zikhalidwe zazing'ono, komanso zikhalidwe zapadera za zinyama zimagwiritsidwa ntchito. Phukusi limapangidwa ndi polyethylene ndipo ndioyenera kuyikamo magazi, inoculation ndi kudzipatula kwa mabakiteriya komanso kudera mbewu.

Zinapangidwa koyambirira mu 1887 kaatonde kumene a bacteria katswiri wa bacteius Richard Petri (1852-1921) mothandizidwa ndi Koch, motero amadziwikanso kuti "Banja". Chisoni cha Petri ". Mbale ya Petri ndi osalimba ndipo ayenera kusamalira mosamala mukatsuka mbale zomwe zagwiritsidwa ntchito munthawi yake ndikuwasunga pamalo otetezeka komanso otetezeka.

1. Kusamba mbale za Petri

a) Kutentha: GAWO GLARWARY Iyenera kunyowa m'madzi poyamba kufewetsa ndikusungunuka. GAMVA YATSOPANO Ingoyenera kusinthidwa ndi madzi apampopi musanayambe kugwiritsa ntchito, kenako ndikunyowa mu 5% hydrochloric acid usiku; Wogwiritsa ntchito galasi nthawi zambiri amakhala ndi mapuloteni ambiri ndi mafuta omwe amaphatikizidwa nazo, zomwe sizophweka kuzisamba mutayanika. Chifukwa chake, iyenera kunyowetsedwa m'madzi oyera ndikukulitsa mukangogwiritsa ntchito.

b) Kukhumba: Ikani galasi lonyowa m'madzi, ndipo sankhani mobwerezabwereza ndi burashi yofewa. Siyani malo akufa ndipo pewani kuwononga pansi kutha kwa chipatala. Sambani ndikuwumitsa galasi loyeretsedwa kuti lisatenge.

c) Kusankha: Kutenga kuloza zinthu zomwe zatchulidwazi poyeretsa yankho, lotchedwanso acid yankho, kuti muchotse zinthu zomwe zingakhalepo pamtunda chifukwa cha maxidation a acid. Kusaka sikuyenera kukhala kochepera maola asanu ndi limodzi, nthawi zambiri usanakhale usiku kapena nthawi yayitali. Samalani ndi ziwiya.

d) Mphete: Mapiritsi ayenera kudulidwa ndi madzi mutatha kukwapula komanso kudontha. Kaya ziwiya zimatsukidwa pambuyo poti kutola mwachindunji kumakhudza kuchita bwino kapena kulephera kwa chikhalidwe cha khungu. Sambani ziwiya zozikika. Zingwe zilizonse ziyenera kukhala zobwerezabwereza "madzi" osachepera 15, ndipo pamapeto pake adatsukidwa katatu ndi madzi okwanira kawiri, owuma kapena owuma kapena owuma kuti agwiritse ntchito pambuyo pake.

e) Kusankhira mitengo yotayika ya petri nthawi zambiri kumakhala chosawilitsidwa ndi ma exradiation kapena mankhwala osinthitsa akachoka fakitale.

2. Kugawika kwa mbale za Petri

a) Malinga ndi kugwiritsa ntchito kosiyanasiyana kwa mbale ya Petri, itha kugawidwa mumikhalidwe yam'manja ndi mbale ya mabakiteriterite.

b) Malinga ndi zinthu zosiyanasiyana zopanga, zimagawidwa m'masamba a petri ndi mbale zamagalasi, koma onse awiriwa adaitanitsa tinthu tating'onoting'ono toti tinthu tating'onoting'ono tomwe timasamba timapepala tomwe timayika pulasitiki.

c) Malinga ndi kukula kosiyanasiyana, nthawi zambiri imatha kugawika mbale mbale za 30m, 60mm, 90mm ndi 150mm.

d) Malinga ndi magawo osiyanasiyana, imatha kugawidwa m'masamba olekanitsidwa ndi 2, mbale zitatu zolekanitsidwa, ndi zina zambiri.

E) Zinthu za mbale ya petri imagawika m'magulu awiri, makamaka pulasitiki ndigalasi, ndipo galasi limatha kugwiritsidwa ntchito pazomera, chikhalidwe cha microbchial ndi maselo a nyama amapikisana ndi chikhalidwe. Opumira amatha kupangidwa ndi polyethylene, ndipo pali kugwiritsa ntchito zinthu zingapo komanso zingapo, zomwe ndizoyenera kuyikamo magazi, kukhazikika, komanso kudzipatula kwa mabakiteriya, ndipo itha kugwiritsidwa ntchito pakukulitsa mitengo.

3. Zinthu zikufunika chisamaliro pakugwiritsa ntchito mbale zachikhalidwe

a) Pambuyo poyeretsa ndi kutsuka musanagwiritse ntchito, kaya mbale ya Petri ndi yoyera kapena yosalimbikitsa kwambiri pa ntchitoyi, yomwe ingakhudze Ph ya sing'anga. Ngati pali mankhwala ena, zimalepheretsa kukula kwa mabakiteriya.

b) Zogula zatsopano za Petri ziyenera kudulidwa ndi madzi otentha poyamba, kenako ndikulowetsedwa mu hydrocloric acid ndi gawo lalikulu la 1% kapena 2% kwa maola angapo kuti muchotse zinthu zam'madzi za alkalines.

c) Ngati mukufuna kukhala ndi mabakiteriya, gwiritsani ntchito stration steipt (nthawi zambiri 10 mpaka 5), ​​tcherani ndi masentimita 10 , ndiye kuti, ikani mbale ya Petri imayiyika mu uvuni ndikusunga matenthedwe pafupifupi 120 ° C kwa maola awiri kuti aphe mabakiteriya.

d) Zakudya zosawinda zidutswa za Petri zitha kugwiritsidwa ntchito poyambira komanso chikhalidwe.