Elisa akugulira a Elisa Agal

Onjezani Kuwongolera: Onjezani zitseko zitatu za kuwongolera zoipa, 1 bwino kuwongolera, ndi mikangano ya olamulira.
Kuphatikiza: Zomwe Elisa 96 Well Will Amafunika kugwedezeka kuti isakanikizedwe kowirikiza, kenako ndikuyika mu chipikika cha 37 C C. ndikuyika mu incubrator kapena madzi osamba kwa mphindi 20 za zomwe achite.
Sambani mbale ya Elisa: yeretsani njira yotsuka 15m ndi madzi osungunuka ku voliyumu ya 300ml. Mukatsuka manja, zomwe zili m'matulo a murete muyezo ziyenera kuthiridwa, njira yotsuka iyenera kudzazidwa ndi zitsimezo iyenera kuchitika ndi zitsimezo, kenako ndikugwedezeka kwa mphindi 30 kenako ndikugwedezeka mwamphamvu. Bwerezani izi nthawi 5 kenako ndikuyamba kuwuma. Makina ochapira ayenera kuchitika kasanu, bowo lililonse limalowetsedwa ndi 200U yotsuka yankho kapena imatha kudzazidwa, kenako imayima
Pambuyo mphindi 30, kukhetsa ndi kuwuma.
Onjezani enzyme kulemba yankho: Onjezani ma 100 a enzyme kuyika njira yothetsera vutoli pachitsime chilichonse, ndikutsuka maikolonde pa 37, ndikusambitsa makhosi 5 pambuyo mphindi 20. Kugwiritsa ntchito kusamba kwa maikolongolekolo kumafanana ndi momwe tafotokozera pamwambapa.
Ichi ndi gawo loyamba la zomwe zidachitikazo, zomwe zili chitukuko chazomwe zimachitika: 50Ul ya gawo lapansi limawonjezedwanso bwino, ndipo utoto umapangidwa kwa mphindi 10 mumdima pansi pa 37. Onjezerani yankho lililonse
50ul adasakanikirana kuti asiye zomwezo.
Kugwiritsa ntchito ma bunsen micretiter kuyenera kuchitika pansi pa chisamaliro. Kutsutsidwa ndi kuyeretsa kuyenera kuchitika mosamala pambuyo pogwiritsa ntchito mbale ya microtiter, koma ziyenera kudziwidwa kuti michere ina
Musanachotsere madioni, muyenera kuwerenga malangizo mosamala kuti muwone ngati itha kukhala yopanda chosawilitsidwa pa kutentha kwambiri.