Mavuto osindikizidwa ndi kuipitsidwa m'mitundu yambiri ya cell

Kutsegula ndi kusamalira mbale zamakhalidwe ambiri:

Chikhalidwe cha cell mbale amatsatiranso mfundo ya asepis asepris pochita ntchito maselo. Ntchito zonse ziyenera kukhala zoyenerera komanso zasayansi, ndipo sizingapangitsenso zovuta pazokulira pa cell. Vuto lofala kwambiri ndi momwe mungapangire kufanana kwa maselo atatha kuwonjezera zitsanzo ndikuchepetsa kusintha kwa sing'anga pokula.

Funsani:
Zingwe za ma 96 ndi 24-Chabwino

Yankho:
Chivindikirocho chimangosungulumwa kwambiri, chomwe ndi chachikhalidwe chotseguka, ndipo cholinga chake ndichakuti, ndikupanga co2 kunja kwa zikhalidwe ndi kusamalira bwino zikhalidwe sing'anga).
Chilichonse chili ndi ubwino ndi zovuta zomwe zimapangitsa, zomwe zimapangitsa kuti ziwonongeke. Kuphatikiza apo, izi zimapangitsa madzi m'mbale kuti apukusa, yomwe ndi yodziwika bwino chifukwa chogwiritsa ntchito mankhwala osokoneza bongo. Chifukwa chake, njira ziwiri zotsatirazi ndizofunikira: a. Mlengalenga mu chofungatira ziyenera kukhala zoyera (zowoneka bwino za ultraviolet, zakumwa za ultraviolet, ndipo zotchinga ziyenera kutsegulidwa ndikutsekedwa pang'ono momwe zingathere) b. Chinyezi mu chofungatira chimayenera kusungidwa nthawi zonse ku 100% (kuyanika ndi madzi osabala omwe adayikidwa mu chofungatira).
Monga mbale ya petri, ndi chidebe chokhala ndi chivundikiro pansi-pansi, ndipo sichidzaipitsidwa. Makamaka chifukwa cha zovuta zoyipa zomwe zimapangidwa ndi "L" m'mphepete mwa chivundikiro, pali ma microorganisms ophatikizidwa ndi fumbi, ndipo fumbi lomwe limanyamulidwa ndi mpweya womwe umapangitsa kuti pakhale zovuta. Mphamvu zolimbitsa mpweya zimangophatikizidwa ndi mpweya, ndipo palibe mpweya wopangidwa, kotero zimangopumira komanso osati kulowa mabakiteriya.

Funsani:
Pogwiritsa ntchito mbale 24, pali ntchito zina zitsime zina (mu benchi yoyera), ndipo ma cell alipo kuti azikhala zitsime zina. Ndili ndi nkhawa kuti izi zibweretsa kuipitsidwa. Sindikudziwa choti nditchere?

Yankho:
Ngati opaleshoniyo yathetsedwa pa benchi yoyera, iyenera kukhala yabwino. Ndikuganiza kuti mutha kugwiritsa ntchito chivundikiro cha mbale yachikhalidwe, yesani kuvumbula mabowo okha kuti agwiritsidwe ntchito, ndikuphimba mabowo enawo ndi zophimba.
Onani kuti musanagwiritse ntchito ndi kugwiritsa ntchito mabowo onse; Ngati mukungofunika kugwiritsa ntchito mabowo ochepa, mutha kugwiritsa ntchito mbali imodzi yokha, ndikuphimba zina zonse ndi chivundikiro. Ndimagwiritsidwa ntchito kugwiritsa ntchito bowo loyenera (ndizotheka kuwonjezera zitsanzo kudzanja lamanja).

Mukamagwira ntchito, gwiritsani ntchito magalasi ochepa kuti akweze mbali imodzi ya bolodi, musatsegule chivundikiro kwathunthu, nthawi zambiri palibe vuto.
Kugawa kwa cell ndi mayankho

Funsani:
Maselo amalandidwa pachipatala, ndipo maselo nthawi zonse amasonkhana mu gawo la gawo, momwe angathane nalo?

Yankho:
Kodi ndingafunse kuti maselo anu asakanizidwe? Kodi ndikupaka kapena kugwedeza mbale ya chikhalidwe? Ngati ili yotsatirayi, ndipo ngati ikugwedezeka pabwalo, ndizotheka kuti maselowo adzaponyedwa ku gawo lalikulu chifukwa cha mphamvu ya centrifugal, chifukwa ma cell ochepa pakati. Opitilira Masabata Awiri!
Nayi njira yabwino: musanayambe kulima mbale ya mbewu, ikani chipatalacho mu chofungatira kwa maola angapo a kutalika ndikuchotsa. Mphamvu iyenera kukhala yowala mukadzala maselo. Kuphatikiza pang'onopang'ono kumalola kuyimitsidwa kwa maselo kuti ayende bwino kumatuka a mbale, ndipo maselo odziwika bwino amakula kwambiri. Kumbukirani kuti musagwedezeke ndi shaker, kapena maselo anu kudzakhala palimodzi monga momwe mudanenera.
Zocheperako dzenje m'mimba mwa anthu, zodziwikiratu izi ndizomwe zimachitika. Izi sizingafanane ndi mbale 24 ndi 96, chifukwa madziwo amatsatira khoma kuti chikhalidwecho chithetsetsekera mu chitsime sichikupanga madzi, koma otumphuka ndi okwera. Komabe, mukamagwiritsa ntchito mitundu iwiriyi ya mathilawa, sizotheka kuwonjezera njira yothetsera vuto chifukwa chakufunika kowonjezereka, kuti maselowo awonekere "m'mphepete mwa chikhalidwe. Zimatengera zomwe zisonyezo muyenera kutsatira. Ngati ndi MT, Immunohistoche akhudze zotsatira chifukwa cha kuchuluka kwa maselo.
Mukamagaya maselo, samalani kuti apewe moyenera kupewa ma cell, ndipo kuchuluka kwa chikhalidwe cha chinsinsi chiyenera kukhala chokwanira. Nthawi zambiri, onjezerani chikhalidwe chokwanira cha chikhalidwe mukayamba, ndikusintha yankho mukawonjezera njira yowonjezera. Kuchuluka kwa chikhalidwe chowonjezera ku njira yothetsera njirayi ndikofanana ndi kuchuluka kwa njira yothetsera chikhalidwe panthawi yofuula, pankhaniyi, chodabwitsa cha "m'mphepete" chidzakonzekeretsa.

Funsani:
Zomwe ndinachita zinali zoyesa zopanga. Ndikofunika kufalitsa maselo mu yunifolomu. Mabowo ambiri ali bwino pamene kachilomboka kamakhala kovomerezeka, ndipo ena a iwo sadziwa yunifolomu. Pali kusiyana kwakukulu mu gulu la cell. Ndikufuna kukufunsani njira iti yomwe mumagwiritsa ntchito powonjezera maselo?

Yankho:
Ndiwovuta kuti pikilo ikhale yoyimitsidwa mu cell. Onetsetsani kuti kuchuluka kwa maselo pachitsime chilichonse kumakhala kosasintha mukamagawana mbale!
Inde, palinso makonzedwe. Kufanana pakati pa zitsime ndikofunikanso. Mwachitsanzo, powonjezera mankhwala, sakanizani mankhwalawo pambuyo pa kutsimikizika kuti muwonetsetse kuti chitsime chilichonse chimakhala chosasintha.
Kuphatikiza apo, powonjezera ma cell ndi mankhwala osokoneza bongo, gulu loyesa ndi gulu lowongolera liyenera kuwonjezeredwanso kuti mupewe kusiyana kwa cell ndi mankhwala osokoneza bongo a kuwonjezera zitsanzo!

Funsani:
Kuwomba uku kuli kale yunifolomu, koma mabowo, mabowo 6, ndipo mabowo 24 amakhala osagwirizana nthawi zonse, ndipo pang'ono pakati. Kuphatikiza apo, zikuwonekeratu kuti kuwerengera kwachitika, ndipo patatha tsiku limodzi kapena awiri, kachulukidwe ka dzenje lililonse ndi kosiyana, komwe kungakhudze kupezeka. Pali njira yabwino?

Yankho:
Ngati mugwiritsa ntchito pipitte pasteur, osayamwa kwambiri nthawi iliyonse, chifukwa maselo mu pipette amangoimira, nthawi zambiri ma cell mu mabowo ochepa ndi akuluakulu, ndipo madziwo atero Tsatirani njira ya S-mawonekedwe.
Ngati mumagwiritsa ntchito pipette, malangizowo ayenera kukonzedwa ndikuchotsedwa kuti apewe kuwononga maselo, kuti nthawi iliyonse yamadzimadzi yatengedwa ikufanana ndi chitsime. Ndizolondola kwambiri kuwonjezera bowo limodzi ndi nsonga. Komanso samalani kusakaniza kuyimitsidwa.

Kukhazikitsa gulu lofanana, n liyenera kukhala lalikulu mokwanira (mutha kuwerengera).

Osamagwedeza mutatha kupanga, popeza kugwedezeka kumayambitsa maselo kuti ayambe kulowera pakatikati. Ndikwabwino kukonzekera kamodzi.

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